Osong Public Health and Research Perspectives

Original Articles

Analysis of Resistance to Macrolide–Lincosamide–Streptogramin B Among mecA-Positive Staphylococcus Aureus Isolates: Mahmoud Khodabandeh, Mohsen Mohammadi, Mohammad Reza Abdolsalehi, Azadeh Alvandimanesh, Mehrdad Gholami, Meysam Hasannejad Bibalan, Abazar Pournajaf, Ramin Kafshgari, Ramazan Rajabnia; Osong Public Health Res Perspect. 2019;10(1):25-31. Published online February 28, 2019; DOI: https://doi.org/10.24171/j.phrp.2019.10.1.06

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Objectives

Genetic determinants conferring resistance to macrolide, lincosamide, and streptogramin B (MLS_B) via ribosomal modification such as, erm, msrA/B and ereA/B genes are distributed in bacteria. The main goals of this work were to evaluate the dissemination of MLS_B resistance phenotypes and genotypes in methicillin-resistant Staphylococcus aureus (MRSA) isolates collected from clinical samples.

Methods

A total of 106 MRSA isolates were studied. Isolates were recovered from 3 hospitals in Tehran between May 2016 to July 2017. The prevalence of MLS_B-resistant strains were determined by D-test, and then M-PCR was performed to identify genes encoding resistance to macrolides, lincosamides, and streptogramins in the tested isolates.

Results

The frequency of constitutive resistance MLS_B, inducible resistance MLS_B and MS_B resistance were 56.2%, 22.9%, and 16.6%, respectively. Of 11 isolates with the inducible resistance MLS_B phenotype, ermC, ermB, ermA and ereA were positive in 81.8%, 63.6%, 54.5% and 18.2% of these isolates, respectively. In isolates with the constitutive resistance MLS_B phenotype, the prevalence of ermA, ermB, ermC, msrA, msrB, ereA and ereB were 25.9%, 18.5%, 44.4%, 0.0%, 0.0%, 11.1% and 0.0%, respectively.

Conclusion

Clindamycin is commonly administered in severe MRSA infections depending upon the antimicrobial susceptibility findings. This study showed that the D-test should be used as an obligatory method in routine disk diffusion assay to detect inducible clindamycin resistance in MRSA so that effective antibiotic treatment can be provided.

Citations

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Zahra Jahanbakhshi, Jamileh Nowroozi, Zahra Kahrarian, Azin Tariniya Gilani, Mohadeseh Ahmadvand, Nasrollah Sohrabi
Journal of Clinical Research in Paramedical Scienc.2024;[Epub] CrossRef
Antimicrobial Resistance and the Prevalence of the Panton-Valentine Leukocidin Gene among Clinical Isolates of Staphylococcus aureus in Lithuania
Agnė Kirkliauskienė, Jonas Kriščiūnas, Jolanta Miciulevičienė, Daiva Radzišauskienė, Tomas Kačergius, Maksim Bratchikov, Lina Kaplerienė
Polish Journal of Microbiology.2024; 73(1): 21. CrossRef
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Shih-Cheng Chang, Jazon Harl Hidrosollo, Lee-Chung Lin, Yu-Hsiang Ou, Cheng-Yen Kao, Jang-Jih Lu
Journal of Microbiology, Immunology and Infection.2023; 56(2): 292. CrossRef
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Yi-Fei Wang, Tian-Gui Cai, Zhe-Lun Liu, Hui-Ling Cui, Dong Zhu, Min Qiao
Journal of Hazardous Materials.2023; 451: 131133. CrossRef
Phenotypic and Genotypic Characterization of Macrolide-Lincosamide-Streptogramin Resistance in Staphylococcus aureus Isolates from Bovine and Human
Ozgul Gulaydin, Kemal Gurturk, Ismail Hakki Ekin, Ziya Ilhan, Cigdem Arabaci
Acta Veterinaria.2023; 73(1): 102. CrossRef
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Yujie Zhang, Ai Kitazumi, Yen-Te Liao, Benildo G. de los Reyes, Vivian C. H. Wu, Thomas G. Denes
Microbiology Spectrum.2023;[Epub] CrossRef
New update on molecular diversity of clinical Staphylococcus aureus isolates in Iran: antimicrobial resistance, adhesion and virulence factors, biofilm formation and SCCmec typing
Mahtab Tabandeh, Hami Kaboosi, Mojtaba Taghizadeh Armaki, Abazar Pournajaf, Fatemeh Peyravii Ghadikolaii
Molecular Biology Reports.2022; 49(4): 3099. CrossRef
Inducible Clindamycin-Resistant Staphylococcus aureus Strains in Africa: A Systematic Review
Muluneh Assefa, Faham Khamesipour
International Journal of Microbiology.2022; 2022: 1. CrossRef
Genetic Diversity and Virulence Profile of Methicillin and Inducible Clindamycin-Resistant Staphylococcus aureus Isolates in Western Algeria
Zahoua Mentfakh Laceb, Seydina M. Diene, Rym Lalaoui, Mabrouk Kihal, Fella Hamaidi Chergui, Jean-Marc Rolain, Linda Hadjadj
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Xin-rong Pan, Lei Chen, Li-ping Zhang, Jian-e Zuo
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Masoumeh Navidinia, Anis Mohammadi, Reza Arjmand, Masoud Dadashi, Mehdi Goudarzi
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Molecular characterization of invasive Staphylococcus aureus strains isolated from patients with diabetes in Iran: USA300 emerges as the major type
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Joel Manyahi, Sabrina J. Moyo, Said Aboud, Nina Langeland, Bjørn Blomberg
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Malihe Soltani, Bahareh Hajikhani, Samira Zamani, Mehrdad Haghighi, Ali Hashemi, Mohammad Javad Nasiri, Masoud Dadashi, Behzad Pourhossein, Mehdi Goudarzi
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Mojtaba Memariani, Hamed Memariani, Hamideh Moravvej
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Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized Patients
Amr S. Bishr, Salma M. Abdelaziz, Ibrahim S. Yahia, Mahmoud A. Yassien, Nadia A. Hassouna, Khaled M. Aboshanab
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Relationship between MLSB resistance and the prevalent virulence genotypes among Bulgarian Staphylococcus aureus isolates
Virna-Maria Tsitou, Ivan Mitov, Raina Gergova
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Giuseppe Mancuso, Angelina Midiri, Elisabetta Gerace, Carmelo Biondo
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Maria Miklasińska-Majdanik
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Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Human Bloodstream Infections: Detection of MLSB Resistance
Vanessa Silva, Sara Hermenegildo, Catarina Ferreira, Célia M. Manaia, Rosa Capita, Carlos Alonso-Calleja, Isabel Carvalho, José Eduardo Pereira, Luis Maltez, José L. Capelo, Gilberto Igrejas, Patrícia Poeta
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Resistance profile to antimicrobials agents in methicillin-resistant Staphylococcus aureus isolated from hospitals in South Brazil between 2014-2019
Adriana Medianeira Rossato, Muriel Primon-Barros, Lisiane da Luz Rocha, Keli Cristine Reiter, Cícero Armídio Gomes Dias, Pedro Alves d’Azevedo
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A Novel PCR Assay for Detecting Brucella abortus and Brucella melitensis: Saeed Alamian, Majid Esmaelizad, Taghi Zahraei, Afshar Etemadi, Mohsen Mohammadi, Davoud Afshar, Soheila Ghaderi; Osong Public Health Res Perspect. 2017;8(1):65-70. Published online February 28, 2017; DOI: https://doi.org/10.24171/j.phrp.2017.8.1.09

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Abstract PDF

Objectives

Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify Brucella spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis.

Methods

All complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all available Brucella sequences. A unique repeat sequence (URS) locus on chromosome 1 could differentiate Brucella abortus from Brucella melitensis. A primer set was designed to flank the unique locus. A total of 136 lymph nodes and blood samples were evaluated and classified by the URS-polymerase chain reaction (PCR) method in 2013–2014.

Results

Biochemical tests and bacteriophage typing as the golden standard indicated that all Brucella spp. isolates were B. melitensis biovar 1 and B. abortus biovar 3. The PCR results were the same as the bacteriological method for detecting Brucella spp. The sensitivity and specificity of the URS-PCR method make it suitable for detecting B. abortus and B. melitensis.

Conclusion

Quick detection of B. abortus and B. melitensis can provide the most effective strategies for control of these bacteria. The advantage of this method over other presented methods is that both B. abortus and B. melitensis are detectable in a single test tube. Furthermore, this method covered 100% of all B. melitensis and B. abortus biotypes. The development of this URS-PCR method is the first step toward the development of a novel kit for the molecular identification of B. abortus and B. melitensis.

Citations

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Development of a simplified and cost-effective sample preparation method for genotyping of human papillomavirus by next-generation sequencing
Rungrat Jitvaropas, Ukrit Thongpoom, Vorthon Sawaswong, Kritsada Khongnomnan, Witthaya Poomipak, Kesmanee Praianantathavorn, Pornjarim Nilyanimit, Yong Poovorawan, Sunchai Payungporn
Archives of Virology.2023;[Epub] CrossRef
Bovine brucellosis – a comprehensive review
Sandip Kumar Khurana, Anju Sehrawat, Ruchi Tiwari, Minakshi Prasad, Baldev Gulati, Muhammad Zubair Shabbir, Rajesh Chhabra, Kumaragurubaran Karthik, Shailesh Kumar Patel, Mamta Pathak, Mohd. Iqbal Yatoo, Vivek Kumar Gupta, Kuldeep Dhama, Ranjit Sah, Wanpe
Veterinary Quarterly.2021; 41(1): 61. CrossRef
Survey of Zoonotic Bacterial Pathogens in Native Foxes in Central Chile: First Record of Brucella canis Exposure
Nicolás Galarce, Sebastián de la Fuente, Beatriz Escobar, Phillip Dettleff, Pedro Abalos, Juan Carlos Hormazábal, Roberto Flores, Nicole Sallaberry-Pincheira, Víctor Martínez
Animals.2021; 11(7): 1980. CrossRef
Development and validation of immunoassay for whole cell detection of Brucella abortus and Brucella melitensis
Richa Hans, Pranjal Kumar Yadav, Pushpendra Kumar Sharma, Mannan Boopathi, Duraipandian Thavaselvam
Scientific Reports.2020;[Epub] CrossRef
Laboratory Diagnostic Procedures for Human Brucellosis: An Overview of Existing Approaches
Afshar Etemadi, Rezvan Moniri, Heinrich Neubauer, Yasaman Dasteh Goli, Saeed Alamian
Jundishapur Journal of Microbiology.2019;[Epub] CrossRef
Comparison of PCR-RFLP and PFGE for determining the clonality of Brucella isolates from human and livestock specimens
Nasrin Bahmani, Reza Mirnejad, Mohammad Reza Arabestani, Parviz Mohajerie, Seyed Hamid Hashemi, Manoochehr Karami, Mohammad Yousef Alikhani
Saudi Journal of Biological Sciences.2019; 26(2): 256. CrossRef
Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles
Arash Shams, Bahareh Rahimian Zarif, Mojtaba Salouti, Reza Shapouri, Sako Mirzaii
Artificial Cells, Nanomedicine, and Biotechnology.2019; 47(1): 2562. CrossRef
Identification of Brucella genus and eight Brucella species by Luminex bead-based suspension array
Tina S. Lusk Pfefer, Ruth Timme, Julie A. Kase
Food Microbiology.2018; 70: 113. CrossRef

Rapid Molecular Approach for Simultaneous Detection of Salmonella spp., Shigella spp., and Vibrio cholera: Reza Ranjbar, Ali Naghoni, Davoud Afshar, Farhad Nikkhahi, Mohsen Mohammadi; Osong Public Health Res Perspect. 2016;7(6):373-377. Published online December 31, 2016; DOI: https://doi.org/10.1016/j.phrp.2016.10.002

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Abstract PDF

Objectives
Gastrointestinal tract infection is still one of the serious public health problems in many geographic areas and is endemic in most countries including Iran. Early detection of the gastrointestinal tract pathogens can be extremely important. The aim of the current study was to apply a shortened time-multiplex polymerase chain reaction (PCR) for rapid and simultaneous detection of Salmonella spp., Shigella spp., and Vibrio cholera.
Methods
The standard and clinical strains of Salmonella spp., Shigella spp., and V. cholerae were used in the assay. Multiplex PCR was performed and optimized based on amplification of invA, putative integrase, and ompW genes for detecting Salmonella spp., Shigella spp., and V. cholerae, respectively. The specificity of the assay was evaluated by testing 12 different bacterial species.
Results
Only Salmonella spp., Shigella spp., and V. cholerae strains had positive results when subjected to the assay using multiplex PCR. The assay showed a high sensitivity, and no amplification products were observed in multiplex PCR with any of the other microorganisms.
Conclusion
Our study indicated that the invA, putative integrase, and ompW-based multiplex PCR assay appears to be an efficient method for rapid and simultaneous detection of Salmonella spp., Shigella spp., and V. cholerae.

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